The motor protein kinesin uses ATP (adenosinetriphosphate) as a fuel and walks along the microtubule track, carrying out critical tasks such as intracellular transport and cell division. I will explain atomic simulations and single-molecule experiments analyzing modular design in kinesin's mechanochemistry. Strategies for using these findings to study multi-motor behavior will be discussed, including cooperative transport and microtubule organization in mitotic spindle dynamics.