Uncoupled, independent fluxes of water and of 2 solutes, across a membrane separating 2 stirred tanks of equal elasticity.

## Description

Uncoupled, independent fluxes of water and of 2 solutes, A and B, across a membrane separating 2 stirred tanks. Solute activities are assumed unity so concentrations = thermodynamic activity. The model describes a situation similar to that for the simplest expressions of Kedem and Katchalsky (1958) but omits all interactions between solutes and between water and any solute. One can think of the solutes passing though the membrane by passive permeation with permeability coefficients PermA and Perm B, and the water passing through aqueous pores with filtration coefficient or hydraulic conductivity, Lp. The aqueous pores do not permit solute passage. Lp is the same as the traditional filtration coefficient Kf. Lp translates to a conventional permeability for water filtration coefficienr, Pf cm/s, Pf = Lp* Vw / RT where RT = 19.347*10^6 mmHg*cm^3*mol^(-1) at 37C, Vw is the partial molar volume of water, 18 ml/mol or the concentration of water in water is 55.55 M The driving forces are the pressure difference for water flux and the concentration for the solute fluxes. The pressure difference across the membrane is the hydrostatic pressure difference minus the osmotic pressure difference. The osmotic pressure is given by Van't Hoff's Eq: p_osm = a.C.RT, where p_osm is the osmotic pressure, mmHg, "a" is the activity coefficient, assumed in this model to equal unity, C is concentration, M, and RT is the Gas Constant times Temperature Kelvin. In this model the solute doesn't permeate the aqueous pore so there is no consideration of a reflection coefficient, or rather it is assumed to be unity. Thus solute concentration in the pore water is zero, andthere is no solute advection.. The system is composed of two volumes of pressure-dependent size, yet stirred instantaneously continually. The pressure/volume relationship is expressed via the elasticity of the chambers, Elast, the slope of the pressure/volume relationship. An equivalent structure is to use rigid chambers from each of which there rises narrow columns of fluid to heights h1 and h2. The fluid in the columns is considered to be instantaneously mixed with that in the chamber from which it rises. Fluid or volume flux, Jv, from side 1 to side 2 raises difference in the column heighta between the two sides by Base*(h2-h1) = Jv, where Base = area of the base of the column, and the pressure difference rises to (h2-h1)*rho cm H2O, where rho is the fluid density. g/ml. The linear chamber elastance used in this model, Elast mmHg/ml, gives an equivalent measure for flexible chambers, assuming a linear relationship between the pressure change and the volume change. (1 mmHg = 13.59 cm H2O.) Notes: Situation 1:= Model parameter set: par1 PermA = 0, PermB > 0.1. See Notes. Situation 2 = Model parameter set: par2 PermB > 0. See Notes tab for more discussion.

**Figure:** Top shows concentration of solute A and solute B as a function of time (A1, B1: conc in volume 1, A2, B2 conc in volume 2). Note change in volume 1 (V1) from initial volume of 1 ml. Bottom figure shows hydrostatic pressure in V1 and volume 2 (V2) as a function of time. Permeability of A into V2 is zero and A2_{init} and B1_{init} are 0 mM for both figures.

## Equations

The equations for this model may be viewed by running the JSim model applet and clicking on the Source tab at the bottom left of JSim's Run Time graphical user interface. The equations are written in JSim's Mathematical Modeling Language (MML). See the Introduction to MML and the MML Reference Manual. Additional documentation for MML can be found by using the search option at the Physiome home page.

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Katchalsky A and Curran PF. Nonequilibrium Thermodynamics in Biophysics. Cambridge, MA: Harvard University Press, 1965. Kedem O and Katchalsky A. Thermodynamic analysis of the permeability of biological membranes to non-electrolytes. Biochim Biophys Acta 27: 229-246, 1958. Stein WD. The Movement of Molecules across Cell Membranes. New York: Academic Press, 1967. Stein WD. Transport and Diffusion across Cell Membranes. Orlando, Florida: Academic Press Inc., 1986.

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The National Simulation Resource, Director J. B. Bassingthwaighte, Department of Bioengineering, University of Washington, Seattle WA 98195-5061.

**Model development and archiving support at https://www.imagwiki.nibib.nih.gov/physiome provided by the following grants:** NIH U01HL122199 Analyzing the Cardiac Power Grid, 09/15/2015 - 05/31/2020, NIH/NIBIB BE08407 Software Integration, JSim and SBW 6/1/09-5/31/13; NIH/NHLBI T15 HL88516-01 Modeling for Heart, Lung and Blood: From Cell to Organ, 4/1/07-3/31/11; NSF BES-0506477 Adaptive Multi-Scale Model Simulation, 8/15/05-7/31/08; NIH/NHLBI R01 HL073598 Core 3: 3D Imaging and Computer Modeling of the Respiratory Tract, 9/1/04-8/31/09; as well as prior support from NIH/NCRR P41 RR01243 Simulation Resource in Circulatory Mass Transport and Exchange, 12/1/1980-11/30/01 and NIH/NIBIB R01 EB001973 JSim: A Simulation Analysis Platform, 3/1/02-2/28/07.